Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Print this page Email this page Users Online: 116
Year : 2020  |  Volume : 9  |  Issue : 4  |  Page : 222-229

A comparative study of immunofluorescence pattern and line immune assay profile in the detection of the antinuclear antibodies

1 Department of Microbiology and Immunology, Lucid Medical Diagnostics, Kharkhana, Hyderabad, Telangana, India
2 Department of Microbiology and Immunology, Osmania Medical College, Koti, Hyderabad, Telangana, India

Correspondence Address:
Dr. S Y Boorgula
Lucid Medical Diagnostics, Plot no 203, Vasavi Nagar, Kharkhana, Hyderabad - 500 015, Telangana
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and Permissions

Background and Aims: To compare the efficacy of the Immunofluorescence and Line Immune Assay profile in the detection of ANA antibody. To ascertain an association between ANA patterns and the specific antibodies. To correlate the obtained ANA patterns with the Immunoassay. Settings and Design: This cross sectional study was done at a tertiary care center at Hyderabad Telangana. The samples were tested for ANA by Indirect Immunofluorescence (IIF) method and by Line immunoassay (LIA) methods. Materials and Methods: Serum samples of patients with a clinical suspicion of autoimmune disease were tested for ANA by indirect immunofluorescence (IIF) method and Line immunoassay (LIA) during January 2018-December 2018. Serum samples for indirect immunofluorescence were processed in 1:100 dilution using HEp–2010/liver/stomach/kidney biochip (EUROIMMUN AG). The serum samples were further processed for the line immunoassay in 1:100 dilution on nylon strips coated with purified and recombinant antigens as discrete lines with plastic backing coated with the antigens nRNP/Sm, Sm, SSA, Ro-52, SSB, PM-Scl, Scl-70, PCNA, Jo-1, CENP-B, dsDNA, nucleosomes, ribosomal protein-P, histones, anti-mitochondrial antibodies (AMA-M2) along with the control band. The analysis was done by the comparison of the intensity of reaction with the positive control line by image analysis. Results: Out of 236 samples tested, 77 were tested positive for Immunofluorescence and Line immuno assay, 115 were tested negative for Immunofluorescence and Immunoblot assay, only 1 was tested positive for Immunofluorescence and negative for Line immunoassay and 43 were tested positive for Line immunoassay and negative for Immunofluorescence. Conclusion: Detection of ANA by IIF method is a cost effective method. It may be used for the screening purposes for patients with or without clinical criterion for the autoimmune disease in daily clinical practice. The fluorescent pattern can also predict presence of certain specific antibodies in the sera. This may regulate one from requesting for line immunoassay, which is expensive. The Line Immuno assay may be reserved for those patients who need them for prognostic significance.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded232    
    Comments [Add]    
    Cited by others 1    

Recommend this journal